|Infectious Diseases Department, University of Turin, Italy.
Transfusion blood bags were inoculated with less than 0.1 colony-forming
units (CFU) of Yersinia enterocolitica (serotypes O:3, O:5, and
O:9) and Yersinia pseudotuberculosis I per mL and stored at 4 degrees
C for 41 days. During storage, samples were collected periodically
and colony counts of serial dilutions were performed by plating
on tryptic soy agar. After a lag-phase, the growth of Yersinia species
from less than 0.1 CFU per mL to 10(9) CFU per mL was observed in
3 of 10 bags. The data showed that prolonged storage of contaminated
blood bags allows Y. enterocolitica and Y. pseudotuberculosis to
multiply to high levels.
Prevention of leakage of Di-(2-ethylhexyl)phthalate from blood
bags by glow discharge treatment and its effect on aggregability
of stored platelets.
Platelets storage in glow discharge treated PVC bags was studied.
The amount of leaked di-(2-ethylhexyl) phthalate (DEHP) was 150-200
micrograms/ml/day in the nontreated PVC bags, but only 20-40 micrograms/ml
in the treated bags after 48 h. The adhesion of silicone to PVC
was much improved, and consequently, uniform coating with silicone
became feasible. The decrease of the ability of platelets to aggregate
was accelerated by DEHP. When stored platelets were resuspended
in fresh plasma, the ability to aggregate was gradually restored.
However, the degree of restoration of the ability of platelets which
had been incubated with DEHP was low. When platelets were stored
in the glow discharge treated and then silicone-coated PVC bags,
their adhesion on the surface and the decrease of their function
Platelet storage for 7 days in second-generation blood bags
Plastic storage bags designed to optimize O2 and CO2 transfer to
preserve platelets for 7 days prior to transfusion were studied
in vivo and in vitro. Platelets stored 7 days in second-generation
CLX bags were compared to platelets stored 3 days in standard (CL-3861)
3-day storage bags and platelets transfused within 24 hours of collection.
The CLX bags maintained concentrate pH at a mean of 6.85 +/− 0.03
(SEM) after 7 days, while in standard bags after 3 days of storage,
the mean pH was 6.46 +/− 0.03. A smaller proportion of platelets
stored 7 days in CLX bags were discarded because of a pH less than
6.0 compared to those stored 3 days in CL-3861 bags (10 vs 21%).
Poststorage pH showed strong correlation with concentrate platelet
count and weak correlation with concentrate white cell count in
both bag types. There was no significant difference in the mean
corrected platelet count increments between platelets stored 7 days
in second generation CLX bags and those stored 3 days in CL-3861
bags (10,000 and 12,200 at 1 hour, and 7000 and 7500 at 24 hours,
respectively) following transfusion to 16 thrombocytopenic recipients.
However, transfusion of fresh platelets achieved mean corrected
increments at both 1 and 24 hours posttransfusion that were higher
than seen with either group of stored platelets (20,100 at 1 hour
and 10,800 at 24 hours). Platelets can be stored 7 days in second-generation
CLX blood bags with results comparable to those of platelets stored
3 days in standard bags.